Summary

A large number of laboratory techniques involving DNA are based around the technique of electrophoresis, which sorts fragments of DNA into “bands” of different sizes. In this exercise, you will use enzymes (restriction endonucleases) which cut the DNA strand at specific sequences to drop out a gene which has been inserted into a circular length of DNA (a plasmid) and then demonstrate both this insert and the plasmid using agarose gel electrophoresis.

Brief (one page) protocol

View the online manual in the section below or download [.pdf, 600kb]

Risk assessments

  • DNA Electrophoresis Risk Assessment [download .pdf - 231KB]

Supplementary information