Our cells are chock full of proteins - e.g. enzymes, receptors, structural proteins, signaling proteins - which carry out all the various cellular activities. In this workshop, we will examine how the structure of proteins determines their function, and how temperature can impact on protein structure and function. You will use Green Fluorescent Protein (GFP) as your model protein and employ routine biochemical methods like column chromatography and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) to purify and analyse your protein of interest.

Brief (one page) Protocol

Risk assessments

  • Purification of GFP protein [download .pdf 100kb]
  • SDS-polyacrylamide gel electrophoresis [download .pdf 302kb]

Supplementary information