Cell biologists use microscopes to help them study cells and the structures within them. Routine light microscopy is capable of visualising large components, such as the nucleus, nucleoli and vacuoles, however it is limited in demonstrating smaller structures, or the distribution of specific proteins within the cell. Immunofluorescence is a technique which uses labelled antibodies to demonstrate subcellular structures. In this workshop, you will be using cells from a cancer cell line which expresses the jellyfish green fluorescent protein (GFP) in their nucleus. In addition, you will be using immunofluorescence to demonstrate the cytoskeletal protein tubulin and Complex IV (Cytochrome C Oxidase), a protein found in the membranes of mitochondria.

Brief (one page) Protocol

View the online manual in the section below or download (.pdf, 1.6Mb)

Risk assessments

  • Working in a PC2 Laboratory Risk Assessment [download .pdf 204kb]
  • Mammalian Tissue Culture Risk Assessment [download .pdf 272kb]
  • Immunofluorescence Risk Assessment [download .pdf 235kb]

Supplementary information