This year, one of our themes will be the use of fluorescent proteins in Cell and Molecular Biology. Students will express Green Fluorescent Protein (GFP) in bacterial or mammalian cells, purify the protein by ion-exchange chromatography, and analyse the protein by SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE). They will also perform DNA plasmid extraction from bacteria by alkaline lysis, followed by analysis of the purified DNA by agarose gel electrophoresis. Mammalian cells expressing GFP will also be examined by fluorescence microscopy.

GFP

GFP

GFP